MFGE8 Protein (His tag)

Produktnummer ABIN7317741

Produktdetails

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses MFGE8 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human MFG-E8/lactadherin/MFGE8 Protein (His Tag)(Active)

Sequenz: Met 1-Cys 387

Produktmerkmale: A DNA sequence encoding the human MFGE8 isoform 1 (Q08431-1) (Met 1-Cys 387) was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: When 5 x 10E4 cells/well are added to Recombinant Human MFG-E8 coated plates (12.5 μg/mL, 100 μL/well), 45-85% cells will adhere after 1 hour at 37°C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: MFG-E8/lactadherin/MFGE8 (MFGE8 Produkte)

Synonyme: BA46 Protein, EDIL1 Protein, HMFG Protein, HsT19888 Protein, MFG-E8 Protein, MFGM Protein, OAcGD3S Protein, SED1 Protein, SPAG10 Protein, hP47 Protein, ba46 Protein, edil1 Protein, oacgd3s Protein, MGC75581 Protein, hst19888 Protein, MGC145109 Protein, MFGE8 Protein, mfge8 Protein, zgc:154157 Protein, MGC99228 Protein, AA408458 Protein, AI325141 Protein, Mfgm Protein, P47 Protein, lactadherin Protein, AGS Protein, MFGME8 Protein, MFGMP-E8 Protein, im:6895406 Protein, mfge8l Protein, zgc:110846 Protein, milk fat globule-EGF factor 8 protein Protein, milk fat globule-EGF factor 8 protein b Protein, milk fat globule-EGF factor 8 protein L homeolog Protein, milk fat globule-EGF factor 8 protein a Protein, MFGE8 Protein, mfge8 Protein, mfge8b Protein, mfge8.L Protein, Mfge8 Protein, mfge8a Protein

Hintergrund:

Background: MFG-E8, also known as lactadherin and MFGE8, contains 1 EGF-like domain and 2 F5/8 type C domains. It also contains a phosphatidylserine (PS) binding domain, as well as an Arginine-Glycine-Aspartic acid motif, which enables the binding to integrins. It binds PS, which is exposed on the surface of apoptotic cells. MFG-E8 is expressed in mammary epithelial cell surfaces and aortic media. Overexpression of MFG-E8 can be found in several carcinomas. MFG-E8 has an opsonization of the apoptotic cells and binding to integrins on the surface of phagocytic cells. It also mediates the engulfment of the dead cell. MFG-E8 plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. It promotes VEGF-dependent neovascularization and contributes to phagocytic removal of apoptotic cells in many tissues. It also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner.

Synonym: BA46,EDIL1,HMFG,hP47,HsT19888,MFG-E8,MFGM,OAcGD3S,SED1,SPAG10

Molekulargewicht: 42 kDa

Pathways: SARS-CoV-2 Protein Interaktom