LRPAP1 Protein (His tag)

Produktnummer ABIN7320332

Produktdetails

Target: LRPAP1 (Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses LRPAP1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Mouse LRPAP1/A2MRAP Protein (His Tag)(Active)

Sequenz: Gln 29-Leu 360

Produktmerkmale: A DNA sequence encoding the mature form of mouse LRPAP1 (NP_038615.2) extracellular domain (Gln 29-Leu 360) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized mouse LRPAP1 at 0.5 μg/ml can bind human VLDLR with a linear range of 1.28-32 ng/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: LRPAP1 (Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1))

Andere Bezeichnung: LRPAP1/A2MRAP (LRPAP1 Produkte)

Synonyme: wu:fi20f07 Protein, wu:fi22c06 Protein, LRPAP1 Protein, DKFZp459I2430 Protein, A2MRAP Protein, A2RAP Protein, HBP44 Protein, MRAP Protein, RAP Protein, AA617339 Protein, AI790446 Protein, AU042172 Protein, C77774 Protein, alpha-2-macroglobulin receptor-associated protein-like Protein, low density lipoprotein receptor-related protein associated protein 1 Protein, LDL receptor related protein associated protein 1 Protein, LDL receptor related protein associated protein 1 L homeolog Protein, LOC100231803 Protein, lrpap1 Protein, LRPAP1 Protein, Lrpap1 Protein, lrpap1.L Protein

Hintergrund:

Background: Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiosis. The genetic variations at the LRPAP1 locus may modulate Alzheimer disease (AD) phenotype beyond risk for disease. In addition, the variation at the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).

Synonym: AA617339,AI790446,AU042172,C77774,HBP44,RAP

Molekulargewicht: 40.4 kDa

NCBI Accession: NP_038615