Intestinal Alkaline Phosphatase Protein (ALPI) (His tag)
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- Target Alle Intestinal Alkaline Phosphatase (ALPI) Proteine anzeigen
- Intestinal Alkaline Phosphatase (ALPI)
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses Intestinal Alkaline Phosphatase Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)
- Sequenz
- Met 1-Asp 503
- Produktmerkmale
- A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
- Reinheit
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin-Niveau
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1,000 pmoles/min/μg.
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Intestinal Alkaline Phosphatase (ALPI) (AA 74-162) protein (GST tag)
ALPI Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
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Intestinal Alkaline Phosphatase (ALPI) (AA 20-503) protein (His tag)Crystallography grade ALPI Spezies: Human Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Intestinal Alkaline Phosphatase (ALPI) (AA 20-528) protein (His tag)Crystallography grade ALPI Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Intestinal Alkaline Phosphatase (ALPI) protein (Myc-DYKDDDDK Tag)ALPI Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Intestinal Alkaline Phosphatase (ALPI)
- Andere Bezeichnung
- Alkaline Phosphatase/ALPI (ALPI Produkte)
- Synonyme
- IAP Protein, Alp1 Protein, S51097 Protein, 2010001C14Rik Protein, Akp6 Protein, gIAP Protein, alpi Protein, im:7149588 Protein, si:ch211-262h13.1 Protein, wu:fa95f04 Protein, zgc:110794 Protein, ALPI Protein, Alpi Protein, alkaline phosphatase, intestinal Protein, alkaline phosphatase, intestinal, tandem duplicate 1 Protein, intestinal-type alkaline phosphatase Protein, ALPI Protein, Alpi Protein, alpi.1 Protein, LOC100724954 Protein
- Hintergrund
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Background: ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Synonym: IAP
- Molekulargewicht
- 54 kDa
- UniProt
- P09923
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