IL28B Protein (His tag)

Produktnummer ABIN7196532

Produktdetails

Target: IL28B (Interleukin 28B (Interferon, lambda 3) (IL28B))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Hefe

Aufreinigungstag / Konjugat: Dieses IL28B Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human IFN-λ3/IL-28B Protein (Yeast, His Tag)(Active)

Sequenz: Val18-Val196

Produktmerkmale: A DNA sequence encoding the human IFNL3 (NP_742151.2) (Val18-Val196) was expressed with a polyhistidine tag at the C-terminus.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is 8-40ng/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, PH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: IL28B (Interleukin 28B (Interferon, lambda 3) (IL28B))

Andere Bezeichnung: IFN-lambda3/IL-28B (IL28B Produkte)

Synonyme: Il28b Protein, IFL-1 Protein, IL-28B Protein, INF-alpha Protein, INF-lambda Protein, Il28 Protein, IL28B Protein, IL28C Protein, interferon, lambda 3 Protein, interferon lambda 3 Protein, Ifnl3 Protein, IFNL3 Protein

Hintergrund:

Background: Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNλ) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

Synonym: Interleukin-28B, IL-28B, Cytokine Zcyto22, Interferon Lambda-3, IFN-Lambda-3, Interferon Lambda-4, IFN-Lambda-4, Interleukin-28C, IL-28C, IL28B, IFNL3, IFNL4, IL28C, ZCYTO22

Molekulargewicht: 21.3 kDa

NCBI Accession: NP_742151