ESM1 Protein (His tag)

Produktnummer ABIN7317534

Produktdetails

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses ESM1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human ESM1/Endocan Protein (His Tag)(Active)

Sequenz: Met 1-Arg184

Produktmerkmale: A DNA sequence encoding the human ESM1 (NP_008967.1) (Met1-Arg184) was expressed with a C-terminal polyhistidine tag.

Reinheit: > 95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ESM1-His coated plates (10μg/mL, 100 μL/well), approximately 48% will adhere after 30 minutes at 37°C.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Andere Bezeichnung: ESM1/Endocan (ESM1 Produkte)

Synonyme: ESM1 Protein, zgc:153027 Protein, 0610042H23Rik Protein, AV004503 Protein, ESM-1 Protein, Pg25 Protein, endocan Protein, endothelial cell specific molecule 1 Protein, endothelial cell-specific molecule 1 Protein, ESM1 Protein, esm1 Protein, Esm1 Protein

Hintergrund:

Background: ESM1 is a secreted protein which is produced by adipocytes. It has been noticed that ESM1 may play some role in obesity-associated vascular disease since circulating ESM-1 levels are reduced in the overweight and obese. ESM1 is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of ESM1 gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. Recently, ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF or FGF-2. In hypervascularized cancers, overexpression of endocan has been detected by immunohistochemistry using monoclonal antibodies against ESM1.

Synonym: endocan

Molekulargewicht: 19.6 kDa

NCBI Accession: NP_008967

Pathways: Growth Factor Binding