ERAP2 Protein (His tag)

Produktnummer ABIN7195485

Produktdetails

Target: ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses ERAP2 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human LRAP/ERAP2 Protein (His Tag)(Active)

Sequenz: Ala 56-Thr 960

Produktmerkmale: A DNA sequence encoding the lumenal domain of human ERAP2 (NP_071745.1) (Ala 56-Thr 960) was expressed with a polyhistidine tag at the N-terminus.

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC). The specific activity is >50 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, pH 7.5, 50 % glycercol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: ERAP2 (Endoplasmic Reticulum Aminopeptidase 2 (ERAP2))

Andere Bezeichnung: LRAP/ERAP2 (ERAP2 Produkte)

Synonyme: L-RAP Protein, LRAP Protein, ERAP2 Protein, zgc:172163 Protein, endoplasmic reticulum aminopeptidase 2 Protein, ERAP2 Protein, erap2 Protein

Hintergrund:

Background: Leukocyte-derived arginine aminopeptidase (LRAP), also known as endoplasmic reticulum-aminopeptidase 2 (ERAP2), is the second identified aminopeptidase localized in the in the lumenal side of endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. It is a 960-amino acid protein with significant homology to placental leucine aminopeptidase and adipocyte-derived leucine aminopeptidase. LRAP preferentially hydrolyzes the basic residues Arg and Lys, and contains the HEXXH(X)18E zinc-binding motif, which is the characteristic of the M1 family of zinc metallopeptidases which also includes PILS/ARTS1/ERAP1 and LNPEP/PLAP. Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors.

Synonym: FLJ23633,FLJ23701,FLJ23807,L-RAP,LRAP,LRAP.ERAP2

Molekulargewicht: 106 kDa

NCBI Accession: NP_071745