CPM Protein (His tag)

Produktnummer ABIN7194614

Produktdetails

Target: CPM (Carboxypeptidase M (CPM))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CPM Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Carboxypeptidase M/CPM Protein (His Tag)(Active)

Sequenz: Met 1-His 422

Produktmerkmale: A DNA sequence encoding the human CPM (NP_938079.1) without the propeptide (Met 1-His 422) was expressed, fused with a polyhistidine tag at the C-terminus.

Reinheit: > 98 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its ability to release Larginine from BenzoylAlaArg, with detection of the arginine amino group by ophthaldialdehyde.The specific activity is >40,000 pmoles/min/μg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CPM (Carboxypeptidase M (CPM))

Andere Bezeichnung: Carboxypeptidase M/CPM (CPM Produkte)

Synonyme: im:7143022 Protein, im:7143022l Protein, si:dkey-11p6.1 Protein, zgc:110307 Protein, CPM Protein, 1110060I01Rik Protein, 5730456K23Rik Protein, AA589379 Protein, E030045M14Rik Protein, carboxypeptidase M Protein, carboxypeptidase D Protein, CPM Protein, cpm Protein, LOC5574253 Protein, Cpm Protein

Hintergrund:

Background: Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.

Synonym: Carboxypeptidase M,CPM

Molekulargewicht: 47.7 kDa

NCBI Accession: NP_938079