CHST15 Protein (His tag)

Produktnummer ABIN7317684

Produktdetails

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CHST15 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human CHST15/BRAG Protein (His Tag)

Sequenz: Ser 99-Thr 561

Produktmerkmale: A DNA sequence encoding the human BRAG (NP_056976.2) extracellular domain (Ser 99-Thr 561) was expressed, with a polyhistidine tag at the N-terminus.

Reinheit: > 97 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CHST15 (Carbohydrate (N-Acetylgalactosamine 4-Sulfate 6-O) Sulfotransferase 15 (CHST15))

Andere Bezeichnung: CHST15/BRAG (CHST15 Produkte)

Synonyme: GALNAC4S-6ST Protein, BRAG Protein, RP11-47G11.1 Protein, 4631426J05Rik Protein, GalNAcS-6ST Protein, MAd5 Protein, mKIAA0598 Protein, GalNAc4S6ST Protein, Galnac4s-6st Protein, carbohydrate sulfotransferase 15 Protein, carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 Protein, CHST15 Protein, chst15 Protein, Chst15 Protein

Hintergrund:

Background: Carbohydrate sulfotransferase 15, also known as N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase, GalNAc4S-6ST, B-cell RAG-associated gene protein, CHST15 and BRAG, is a single-pass type I I membrane protein which belongs to the sulfotransferase 1 family. CHST15 / BRAG is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It is not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. CHST15 / BRAG is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 / BRAG may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression.

Synonym: BRAG;GALNAC4S-6ST

Molekulargewicht: 56 kDa

NCBI Accession: NP_056976

Pathways: Glycosaminoglycan Metabolic Process