CD57 Protein (His tag)

Produktnummer ABIN7319929

Produktdetails

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Human Cells

Aufreinigungstag / Konjugat: Dieses CD57 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human B3GAT1 (N-6His)

Sequenz: His25-Ile334

Produktmerkmale: Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 is produced by our Mammalian expression system and the target gene encoding His25-Ile334 is expressed with a 6His tag at the N-terminus.

Reinheit: >95 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))

Andere Bezeichnung: B3GAT1 (B3GAT1 Produkte)

Synonyme: CD57 Protein, GLCATP Protein, GLCUATP Protein, HNK1 Protein, LEU7 Protein, NK-1 Protein, NK1 Protein, 0710007K08Rik Protein, AI846286 Protein, GlcAT-P Protein, Glcatp Protein, Hnk1 Protein, Hnk-1 Protein, b3gat1 Protein, si:dkey-22o20.1 Protein, cd57 Protein, glcatp Protein, glcuatp Protein, hnk-1 Protein, hnk1 Protein, leu7 Protein, nk-1 Protein, nk1 Protein, beta-1,3-glucuronyltransferase 1 Protein, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) Protein, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a Protein, beta-1,3-glucuronyltransferase 1 L homeolog Protein, B3GAT1 Protein, B3gat1 Protein, b3gat1a Protein, b3gat1.L Protein

Hintergrund:

Background: B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.

Synonym: B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), CD57, GlcAT-P, HNK1, NK1, NK-1

Molekulargewicht: 36.2 kDa

UniProt: Q9P2W7

Pathways: Glycosaminoglycan Metabolic Process