beta Actin Protein (full length) (rho-1D4 tag)

Produktnummer ABIN7126051

Produktdetails

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: CHO cells

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses beta Actin Protein ist gelabelt mit rho-1D4 tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys), Functional Studies (Func)

Sequenz: MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS TFQEMWISKQ EYDESGPSIV HRKCF
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Actin, cytoplasmic 1 (Beta-actin) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Güteklasse: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung: Actin, cytoplasmic 1 (Beta-actin) (ACTB Produkte)

Synonyme: BRWS1 Protein, PS1TP5BP1 Protein, ACTA1 Protein, Actx Protein, E430023M04Rik Protein, beta-actin Protein, ps1tp5bp1 Protein, act-2 Protein, actb Protein, B-actin Protein, LOC100174883 Protein, LOC100304816 Protein, A Protein, ACT5C Protein, Ac5C Protein, Act Protein, Act-5C Protein, Act5 Protein, Act5c Protein, Actin Protein, Actin/BAP47 Protein, Actin5C Protein, BAP47 Protein, Bap47 Protein, CG4027 Protein, Dmel\\CG4027 Protein, M32055 Protein, T11 Protein, act Protein, act 5C Protein, act5C Protein, actin Protein, actin5C Protein, anon-EST:fe2D2 Protein, beta-actin/Bap47 Protein, cyt5C Protein, l(1)G0009 Protein, l(1)G0010 Protein, l(1)G0025 Protein, l(1)G0079 Protein, l(1)G0117 Protein, l(1)G0177 Protein, l(1)G0245 Protein, l(1)G0330 Protein, l(1)G0420 Protein, l(1)G0486 Protein, Bact Protein, ACT-5 Protein, ACTB Protein, B-ACTZF Protein, actba Protein, bact Protein, bactin1 Protein, bactzf Protein, wu:fd18f05 Protein, A26C1B Protein, POTE2alpha Protein, POTEACTIN Protein, Beta-actin Protein, actin beta Protein, actin, beta Protein, cytoplasmic actin OlCA1 Protein, beta-actin Protein, Actin 5C Protein, actin gamma 1 Protein, actin, beta 1 Protein, actin, beta L homeolog Protein, beta actin Protein, POTE ankyrin domain family member F Protein, actin, beta like 2 Protein, actin, cytoplasmic 1 Protein, ACTB Protein, Actb Protein, actb Protein, LOC100174883 Protein, LOC100304816 Protein, Act5C Protein, ACTG1 Protein, actb1 Protein, actb.L Protein, LOC100136352 Protein, POTEF Protein, ACTBL2 Protein, LOC109073280 Protein

Hintergrund: Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. {ECO:0000250|UniProtKB:P60709}.

UniProt: P48975

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction