Lipase (full length) protein (rho-1D4 tag)

Produktnummer ABIN7126046

Produktdetails

Target: Lipase

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: CHO cells

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: rho-1D4 tag

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequenz: MQILGLVVCL FLSVLLSGRP TGSIPHVDPE ANMNVTEMIR YWGYPSEEHM IQTEDGYILG VHRIPHGRKN HSHKGPKPVV YLQHGFLADS SNWVTNSDNS SLGFILADAG FDVWLGNSRG NTWSLKHRTL SISQDEFWAF SFDEMAKYDL PASIYYIVNK TGQEQVYYVG HSQGTTIGFI AFSQIPELAK KIKMFFALAP VVFLNFALSP VIKISKWPEV IIEDLFGHKQ FFPQSAKLKW LSTHVCNRVV LKKLCTNVFF LICGFNEKNL NESRVNVYTS HSPAGTSVQN LRHWGQIAKH HMFQAFDWGS KAKNYFHYNQ TCPPVYDLKD MLVPTALWSG DHDWLADPSD VNILLTQIPN LVYHKRLPDW EHLDFLWGLD APWRMYNEIV NLLRKYQ
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Lipase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Güteklasse: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Lipase

Synonyme: EDL Protein, EL Protein, PRO719 Protein, 3110013K01Rik Protein, lipase Protein, mEDL Protein, lipase G, endothelial type Protein, lipase, endothelial Protein, LIPG Protein, Lipg Protein

UniProt: G3HQY6