PLEC Protein (full length) (rho-1D4 tag)

Produktnummer ABIN7126015

Produktdetails

Target: PLEC (Plectin (PLEC))

Protein-Typ: Recombinant

Proteineigenschaft: full length

Spezies: CHO cells

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PLEC Protein ist gelabelt mit rho-1D4 tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)

Sequenz: MVAGMLMPLD QLRAIYEVLF REGVMVAKKD RRPRSLHPHV PGVTNLQVMR AMASLRARGL VRETFAWRHF YWYLTNEGID HLRQYLHLPP EIVPASLQRV RRPVAMVMPA RRRSPHVQSM QGPLSCPPKR GPLPAEDPVR EERKVYRRKE PEEGAPETPV VSATTLGTLA RPGREPAPAT DERDRVQKKT FTKWVNKHLI KAQRHISDLY EDLRDGHNLI SLLEVLSGDS LPREKGRMRF HKLQNVQIAL DYLRHRQVKL VNIRNDDIAD GNPKLTLGLI WTIILHFQIS DIQVSGQSED MTAKEKLLLW SQRMVEGYQG LRCDNFTTSW RDGRLFNAII HRHKPMLIDM NKVYRQTNLE NLDQAFSVAE RDLGVTRLLD PEDVDVPQPD EKSIITYVSS LYDAMPRVPG AQDGVRANEL QLRWQEYREL VLLLLQWIRH HTAAFEERKF PSSFEEIEIL WCQFLKFKET ELPAKEADKN RSKGIYQSLE GAVQAGQLKI PPGYHPLDVE KEWGKLHVAI LEREKQLRSE FERLECLHFI VSKLQMEAGL CEEQLNQADS LLQSDIRLLA SGKVAQRAGE VERDLDKADG MIRLLFNDVQ TLKDGRHPQG EQMYRRVYRL HERLVAIRTE YNLRLKAGVA APVTQVTLQT TQRRPELEDS TLRYLQDLLA WVEENQRRID SAEWGVDLPS VEAQLGSHRG MHQSIEEFRA KIERARNDES
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• CHO Plectin-1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Three step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.

Güteklasse: Crystallography grade

Biological Activity Comment: Protein has not been tested for activity yet.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PLEC (Plectin (PLEC))

Andere Bezeichnung: Plectin-1 (PLEC Produkte)

Synonyme: EBS1 Protein, EBSO Protein, HD1 Protein, LGMD2Q Protein, PCN Protein, PLEC1 Protein, PLEC1b Protein, PLTN Protein, AA591047 Protein, AU042537 Protein, Plec1 Protein, Pcn Protein, Pltn Protein, IFAP300 Protein, Plectin-1 Protein, plectin Protein, plectin L homeolog Protein, LOC100481602 Protein, plec.L Protein, PLEC Protein, Plec Protein, LOC786966 Protein

UniProt: G3HWJ2