SIGLEC7 Protein (Leu117Ala-Mutant, Leu118Ala-Mutant) (Fc Tag)

Produktnummer ABIN7125961

Produktdetails

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Protein-Typ: Recombinant

Proteineigenschaft: Leu117Ala-Mutant, Leu118Ala-Mutant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses SIGLEC7 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human Siglec-7 / CD328 Protein, Fc (L117A, L118A) Tag (MALS verified)

Sequenz: Gln 19 - Leu 353

Produktmerkmale: Human Siglec-7, Fc (L117A, L118A) Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 0.01 EU per μg by the LAL method.

Güteklasse: MALS verified

Anwendungsinformationen

Applikationshinweise: This protein carries a Fc (L117A, L118A) tag at the C-terminus. The protein has a calculated MW of 63.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition due to glycosylation. Mutations (L117A, L118A/EU number: L234A/L235A) in human immunoglobulin G1 (hIgG1) Fc diminish binding to FcγRI leading to a significant reduction in ADCC and CDC in comparison to wild type human IgG1.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 25 mM MES, 150 mM NaCl, pH 5.5

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))

Andere Bezeichnung: Siglec-7 / CD328 (SIGLEC7 Produkte)

Synonyme: SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein

Hintergrund: Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells.

Molekulargewicht: 63.3 kDa

NCBI Accession: NP_055200