Sortilin 1 Protein (SORT1) (His-Avi Tag,Biotin)

Produktnummer ABIN7199468

Produktdetails

Target: Sortilin 1 (SORT1)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Sortilin 1 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human Sortilin / SORT1 Protein, His,Avitag™ (MALS verified)

Sequenz: Ser 78 - Asn 755

Produktmerkmale: Biotinylated Human Sortilin / SORT1, His,Avitag (SON-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ser 78 - Asn 755 (Accession # Q99523-1).

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Güteklasse: MALS verified

Anwendungsinformationen

Applikationshinweise: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 79.3 kDa. The protein migrates as 90-100 kDa under reducing (R) condition due to glycosylation.

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: Sortilin 1 (SORT1)

Andere Bezeichnung: Sortilin / SORT1 (SORT1 Produkte)

Synonyme: SORT1 Protein, 19.m02079 Protein, gp95 Protein, ldlcq6 Protein, sortilin Protein, Gp95 Protein, LDLCQ6 Protein, NT3 Protein, 2900053A11Rik Protein, AI852375 Protein, Ntr3 Protein, Ntsr3 Protein, Nt3 Protein, Nts3 Protein, sort1 Protein, zgc:85953 Protein, sortilin 1 Protein, sortilin Protein, sortilin 1 S homeolog Protein, sortilin 1a Protein, SORT1 Protein, TP01_0016 Protein, BBOV_I002730 Protein, sort1.S Protein, Sort1 Protein, sort1a Protein

Hintergrund: Synonyms: Sortilin,SORT1,100 kDa NT receptor,Glycoprotein 95,Gp95,Gp95LDLCQ6,Neurotensin receptor 3,NT3NTR3,Ntr3,sortilin 1,
Description: The Sortilin is a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Lysosomal proteins bind specifically to the receptor in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelysosomal compartment where the low pH mediates the dissociation of the complex. The receptor is then recycled back to the Golgi for another round of trafficking through its binding to the retromer. Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Molekulargewicht: 79.3 kDa

NCBI Accession: NP_002950

Pathways: Neurotrophin Signalübertragung