CD39 Protein (AA 38-478) (His tag,AVI tag,Biotin)

Produktnummer ABIN6972989

Produktdetails

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 38-478

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD39 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: Biotinylated Human CD39 / ENTPD1 Protein, His,Avitag™ (MALS verified) (active enzyme)

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Applikationshinweise: MALS verified

Kommentare:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0

Lagerung: -80 °C

Antigendetails

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Andere Bezeichnung: CD39 (ENTPD1 Produkte)

Synonyme: ATPDase Protein, CD39 Protein, NTPDase-1 Protein, MGC80631 Protein, zgc:100819 Protein, entpd1 Protein, MGC79567 Protein, 2610206B08Rik Protein, AA408691 Protein, Cd39 Protein, ectonucleoside triphosphate diphosphohydrolase 1 Protein, ectonucleoside triphosphate diphosphohydrolase 1 L homeolog Protein, ENTPD1 Protein, Entpd1 Protein, entpd1.L Protein, entpd1 Protein

Hintergrund: CD39 is also known as Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1, in the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker, but it is also present on the surface of natural killer cells, T cells, and some endothelial cells.Regulatory T cells(Tregs) mediate immunosuppression through multiple, non-redundant, cell-contact dependent and independent mechanisms, a growing body of evidence suggests an important role for the CD39-CD73-adenosine pathway. CD39 ectonucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.

Molekulargewicht: 54.0 kDa