TNF alpha Protein

Produktnummer ABIN6700747

Hersteller Produkt- Nr.: 009-001-306

Dieses Recombinant TNF alpha-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für TNF alpha Protein (ABIN6700747)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: 98,00%

Produktdetails

Hersteller Produkt- Nr.: 009-001-306

Hersteller: Rockland

Verwendungszweck: TNF-α Human Recombinant Protein

Aufreinigung: Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Endotoxin-Niveau: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: TNF-α activity is determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D and is typically less than 0.05 ng/mL.

Anwendungsinformationen

Applikationshinweise:

Application Note: TNFα protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 500 μL

Konzentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Haltbarkeit: 6 months

Antigendetails

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNFA

Hintergrund:

Synonyms: Tumor necrosis factor, TNF-alpha, TNF-a, TNFα, cytokine, Tumor necrosis factor ligand superfamily member 2, Cachectin

Background: TNFα is secreted by macrophages, monocytes, neutrophils, T-cells, and NK- cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha, while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, and GM-CSF. Recombinant Human Tumor Necrosis Factor-alpha produced in E. coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17483.77 Daltons.

UniProt: P01375

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome