PHOSPHO2 Protein (AA 1-241) (His tag)

Produktnummer ABIN668005

Produktdetails

Target: PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-241

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PHOSPHO2 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: PHOSPHO2, 1-241aa, Human, His tag, E.coli

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Lagerung: 4 °C

Antigendetails

Target: PHOSPHO2 (Phosphatase, Orphan 2 (PHOSPHO2))

Andere Bezeichnung: PHOSPHO2 (PHOSPHO2 Produkte)

Synonyme: zgc:172082 Protein, 1700048E23Rik Protein, AI661517 Protein, AU021728 Protein, AV006103 Protein, Phos2 Protein, phosphatase, orphan 2 Protein, phosphatase, orphan 2 L homeolog Protein, PHOSPHO2 Protein, phospho2 Protein, phospho2.L Protein, Phospho2 Protein

Hintergrund: Pyridoxal phosphate phosphatase PHOSPHO2, orphan 2, also known as PHOSPHO2, belongs to the haloacid dehalogenase(HAD) superfamily. Phosphatase has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). PHOSPHO 1, a phosphoethanolamine/phosphocholine phosphatase, is upregulated in mineralising cells and is thought to be involved in the generation of inorganic phosphate for bone mineralization. PHOSPHO2 is a putative phosphatase sharing 42% sequence identity with PHOSPHO1. PHOSPHO1 and PHOSPHO2 are very similar, but surprisingly, recombinant PHOSPHO2 hydrolyses phosphoethanolamine and phosphocholine relatively poorly. Recombinant human PHOSPHO2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Pyridoxal phosphate phosphatase PHOSPHO2, MGC111048, MGC22679, phosphatase orphan 2. NCBI no.: NP_001008489

Molekulargewicht: 30.3 kDa (265aa), confirmed by MALDI-TOF