UBE2D4 Protein (AA 1-147) (His tag)

Produktnummer ABIN667583

Produktdetails

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-147

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses UBE2D4 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Produktmerkmale: UBE2D4, 1-147aa, Human, His tag, E.coli

Reinheit: > 95 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl

Lagerung: 4 °C

Antigendetails

Target: UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))

Andere Bezeichnung: UBE2D4 (UBE2D4 Produkte)

Synonyme: HBUCE1 Protein, UBE2D4 Protein, RGD69425 Protein, Ube2d2 Protein, Ube2d2b Protein, zgc:162263 Protein, ube2d3.2 Protein, EG546638 Protein, Gm5959 Protein, ubiquitin conjugating enzyme E2 D4 (putative) Protein, ubiquitin-conjugating enzyme E2D 4 (putative) Protein, ubiquitin-conjugating enzyme E2 D4 Protein, ubiquitin conjugating enzyme E2D 4 (putative) L homeolog Protein, ubiquitin-conjugating enzyme E2 D2 pseudogene Protein, ubiquitin-conjugating enzyme E2D 4 Protein, UBE2D4 Protein, Ube2d4 Protein, EDI_085450 Protein, EDI_185830 Protein, ube2d4 Protein, ube2d4.L Protein, LOC100354069 Protein

Hintergrund: UBE2D4 belongs to the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, perform the second step in the ubiquitination reaction that targets a protein for degradation via the proteasome. UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Recombinant human UBE2D4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Ubiquitin-conjugating enzyme E2 D4, UBCH5D, HBUCE1. NCBI no.: NP_057067

Molekulargewicht: 18.8kDa (167aa), confirmed by MALDI-TOF