VEGF 165 (AA 207-371) protein (His tag)

Produktnummer ABIN666798

Produktdetails

Target: VEGF 165

Protein-Typ: Recombinant

Proteineigenschaft: AA 207-371

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Produktmerkmale: VEGF165, 207-371aa, Human, His tag, E.coli

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5mg/ml (determined by Bradford assay)

Buffer: Liquid. In 20mM Tris-HCl(pH8.5), 50%glycerol, 5mM DTT, 200mM NaCl, 2mM EDTA

Lagerung: 4 °C

Antigendetails

Target: VEGF 165

Hintergrund: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant human VEGF165 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Synonyms: Vascular endothelial growth factor A isoform d, VPF, VEGF, VEGF-A. NCBI no.: NP_001020539

Molekulargewicht: 21.3kDa (185aa), conformed by MALDI-TOF