Glucuronidase beta Protein (AA 23-651) (His tag)

Produktnummer ABIN7529348

Produktdetails

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 23-651

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses Glucuronidase beta Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFTH HHHHH

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH6.0

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Glucuronidase beta (GUSB) (Glucuronidase, beta (GUSB))

Andere Bezeichnung: GUSB (GUSB Produkte)

Synonyme: GUSB Protein, beta-GUS Protein, gus Protein, BG Protein, MPS7 Protein, AI747421 Protein, Gur Protein, Gus Protein, Gus-r Protein, Gus-s Protein, Gus-t Protein, Gus-u Protein, Gut Protein, asd Protein, g Protein, Ac2-223 Protein, si:ch211-160e1.7 Protein, si:ct573103.7 Protein, glucuronidase beta Protein, beta-D-glucuronidase Protein, beta-glucuronidase Protein, beta glucuronidase Protein, glucuronidase, beta Protein, GUSB Protein, bglR Protein, SSO_RS14735 Protein, Glu Protein, Gusb Protein, gusb Protein

Hintergrund: GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 73.4kDa (635aa) 70-100KDa (SDS-PAGE under reducing conditions.)

NCBI Accession: NP_000172

UniProt: P08236

Pathways: Glycosaminoglycan Metabolic Process