GLUL Protein (AA 1-373) (His tag)

Produktnummer ABIN6387882

Produktdetails

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-373

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GLUL Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer(  pH 8.0) containing 10 % glycerol, 1  mM DTT, 0.1M NaCl

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GLUL (Glutamate-Ammonia Ligase (GLUL))

Andere Bezeichnung: GLUL (GLUL Produkte)

Synonyme: GLNS Protein, GS Protein, PIG43 Protein, PIG59 Protein, Glns Protein, glns Protein, glul Protein, pig43 Protein, pig59 Protein, xgs Protein, p42 Protein, MGC64262 Protein, cb195 Protein, cb757 Protein, etID49849.2 Protein, fb39g09 Protein, fd19d09 Protein, gso1 Protein, sb:cb195 Protein, wu:fb39g09 Protein, wu:fd19d09 Protein, fb11d08 Protein, gso2 Protein, hm:zehn1949 Protein, im:7153900 Protein, wu:fb11d08 Protein, glutamate-ammonia ligase Protein, glutamate-ammonia ligase (glutamine synthetase) Protein, type I glutamate--ammonia ligase Protein, glutamate-ammonia ligase L homeolog Protein, glutamate-ammonia ligase (glutamine synthase) Protein, glutamate-ammonia ligase (glutamine synthase) a Protein, glutamate-ammonia ligase (glutamine synthase) b Protein, GLUL Protein, Glul Protein, AMF_RS09530 Protein, glul.L Protein, glul Protein, CNA04370 Protein, glula Protein, glulb Protein

Hintergrund: GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 23.9 kDa (217aa), confirmed by MALDI-TOF

NCBI Accession: NP_000849

UniProt: Q16774

Pathways: Positive Regulation of Peptide Hormone Secretion