PGAM2 Protein (AA 1-254) (His tag)

Produktnummer ABIN6387878

Produktdetails

Target: PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-254

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PGAM2 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is >100units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PGAM2 (phosphoglycerate Mutase 2 (Muscle) (PGAM2))

Andere Bezeichnung: PGAM2 (PGAM2 Produkte)

Synonyme: zgc:63876 Protein, GSD10 Protein, PGAM-M Protein, PGAMM Protein, D14Mgh1 Protein, Pgmut Protein, phosphoglycerate mutase 2 Protein, phosphoglycerate mutase 2 (muscle) Protein, phosphoglycerate mutase 2 L homeolog Protein, Pgam2 Protein, PGAM2 Protein, pgam2 Protein, pgam2.L Protein

Hintergrund: PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Molekulargewicht: 30.9 kDa (273aa) confirmed by MALDI-TOF

NCBI Accession: NP_000281

UniProt: P15259