PGAM1 Protein (AA 1-254) (His tag)

Produktnummer ABIN6387877

Produktdetails

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-254

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PGAM1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 20 % glycerol, 0.1M NaCl, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PGAM1 (phosphoglycerate Mutase 1 (Brain) (PGAM1))

Andere Bezeichnung: PGAM1 (PGAM1 Produkte)

Synonyme: PGAM-B Protein, PGAMA Protein, Pgmut Protein, MGC81450 Protein, 2310050F24Rik Protein, Pgam-1 Protein, pgam1 Protein, wu:fb81h05 Protein, fd15c02 Protein, pgam1lb Protein, wu:fd15c02 Protein, zgc:63722 Protein, phosphoglycerate mutase 1 Protein, phosphoglycerate mutase 1 L homeolog Protein, phosphoglycerate mutase 1a Protein, phosphoglycerate mutase 1b Protein, PGAM1 Protein, Pgam1 Protein, pgam1.L Protein, pgam1 Protein, pgam1a Protein, pgam1b Protein

Hintergrund: PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Molekulargewicht: 31.4 kDa (278aa) confirmed by MALDI-TOF

NCBI Accession: NP_075907

UniProt: Q9DBJ1

Pathways: Regulation of Carbohydrate Metabolic Process