PGK2 Protein (AA 1-417) (His tag)
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- Target Alle PGK2 Proteine anzeigen
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 1-417
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses PGK2 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Sequenz
- MGSSHHHHHH SSGLVPRGSH MSLSKKLTLD KLDVRGKRVI MRVDFNVPMK KNQITNNQRI KASIPSIKYC LDNGAKAVVL MSHLGRPDGV PMPDKYSLAP VAVELKSLLG KDVLFLKDCV GAEVEKACAN PAPGSVILLE NLRFHVEEEG KGQDPSGKKI KAEPDKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPHKASGFLM KKELDYFAKA LENPVRPFLA ILGGAKVADK IQLIKNMLDK VNEMIIGGGM AYTFLKVLNN MEIGASLFDE EGAKIVKDIM AKAQKNGVRI TFPVDFVTGD KFDENAQVGK ATVASGISPG WMGLDCGPES NKNHAQVVAQ ARLIVWNGPL GVFEWDAFAK GTKALMDEIV KATSKGCITV IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKILPG VEALSNM
- Reinheit
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 500 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.
- Top Product
- Discover our top product PGK2 Protein
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Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (His tag)
PGK2 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (GST tag)PGK2 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 268-339) protein (GST tag)PGK2 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Phosphoglycerate Kinase 2 (PGK2) (AA 1-417) protein (Strep Tag)Crystallography grade PGK2 Spezies: Human Wirt: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Phosphoglycerate Kinase 2 (PGK2) (AA 2-417) protein (His tag)Crystallography grade PGK2 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Phosphoglycerate Kinase 2 (PGK2) protein (Myc-DYKDDDDK Tag)PGK2 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Bioactivity Validated
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.5 mg/mL
- Buffer
- Liquid. 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT, 0.1M NaCl
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- PGK2 (Phosphoglycerate Kinase 2 (PGK2))
- Andere Bezeichnung
- PGK2 (PGK2 Produkte)
- Synonyme
- PGKB Protein, PGKPS Protein, dJ417L20.2 Protein, Pgk-2 Protein, Tcp-2 Protein, PGK2 Protein, phosphoglycerate kinase 2 Protein, PGK2 Protein, Pgk2 Protein, cbbK Protein
- Hintergrund
- PGK2 is a testis-specific form of phosphoglycerate kinase. Initially assumed to be a pseudogene, this protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis. Recombinant human PGK2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molekulargewicht
- 46.9 kDa (437aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_620061
- UniProt
- P07205
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