PGK1 Protein (AA 1-417) (His tag)

Produktnummer ABIN6387707

Produktdetails

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-417

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PGK1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity: > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris (  pH 8.0), containing 10 % Glycerol, 1  mM DTT.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PGK1 (Phosphoglycerate Kinase 1 (PGK1))

Andere Bezeichnung: PGK1 (PGK1 Produkte)

Synonyme: MIG10 Protein, PGKA Protein, Pgk-1 Protein, Pgk Protein, PGK Protein, wu:fd59b07 Protein, wu:fj36g06 Protein, zgc:56252 Protein, zgc:77899 Protein, PGK1 Protein, phosphoglycerate kinase 1 Protein, pgk2 Protein, pgka Protein, phosphoglycerate kinase 1 Protein, phosphoglycerate kinase 1 L homeolog Protein, PGK1 Protein, Pgk1 Protein, pgk1 Protein, LOC100348124 Protein, pgk1.L Protein

Hintergrund: Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 46.8 kDa (437aa), confirmed by MALDI-TOF

NCBI Accession: NP_000282

UniProt: P00558

Pathways: Cellular Glucan Metabolic Process