PGD Protein (AA 1-483) (His tag)

Produktnummer ABIN6387696

Produktdetails

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-483

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses PGD Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0) containing 1  mM DTT, 0.1M Nacl, 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: PGD (Phosphogluconate Dehydrogenase (PGD))

Andere Bezeichnung: PGD (PGD Produkte)

Synonyme: 6PGD Protein, 6-PGD Protein, 6-Pgd Protein, 6-pgd Protein, 6PGDH Protein, 6Pgd Protein, 6Pgdh Protein, 6pgdh Protein, A7 Protein, CG3724 Protein, Dmel\\CG3724 Protein, EG:87B1.4 Protein, N1 Protein, PGD Protein, Pdg Protein, l(1)2Dc Protein, l(1)2De Protein, l(1)A7 Protein, l(1)G0385 Protein, l(1)N1 Protein, l(1)N3[90] Protein, l(1)Pgd Protein, l(1)Pgd-A Protein, l35 Protein, pgd Protein, 0610042A05Rik Protein, AU019875 Protein, C78335 Protein, fi03d03 Protein, zgc:55585 Protein, wu:fi03d03 Protein, zgc:111848 Protein, 6-PGDH Protein, GND1b Protein, Pgd Protein, Cc2-27 Protein, phosphogluconate dehydrogenase Protein, Phosphogluconate dehydrogenase Protein, phosphogluconate dehydrogenase L homeolog Protein, 6-phosphogluconate dehydrogenase, decarboxylating Protein, 6-phosphogluconate dehydrogenase, decarboxylating-like Protein, PGD Protein, Pgd Protein, pgd.L Protein, pgd Protein, GND1a|GND1b Protein, Thal_1580 Protein, GY4MC1_3101 Protein, Odosp_2189 Protein, Marky_1024 Protein, gnd Protein, LOC101451876 Protein

Hintergrund: PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 55.3 kDa (503aa)

NCBI Accession: NP_002622

UniProt: P52209