GSTA4 Protein (AA 1-222) (His tag)

Produktnummer ABIN6387618

Produktdetails

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-222

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GSTA4 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSHMAARPK LHYPNGRGRM ESVRWVLAAA GVEFDEEFLE TKEQLYKLQD GNHLLFQQVP MVEIDGMKLV QTRSILHYIA DKHNLFGKNL KERTLIDMYV EGTLDLLELL IMHPFLKPDD QQKEVVNMAQ KAIIRYFPVF EKILRGHGQS FLVGNQLSLA DVILLQTILA LEEKIPNILS AFPFLQEYTV KLSNIPTIKR FLEPGSKKKP PPDEIYVRTV YNIFRP

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20 mM Tris-HCl buffer (  pH 8.0), 2  mM DTT, 20 % glycerol, 100  mM NaCl

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GSTA4 (Glutathione S-Transferase alpha 4 (GSTA4))

Andere Bezeichnung: GSTA4 (GSTA4 Produkte)

Synonyme: GSTA4-4 Protein, GTA4 Protein, gsta4-4 Protein, gta4 Protein, tGSTA4 Protein, GST5.7 Protein, mGsta4 Protein, glutathione S-transferase alpha 4 Protein, glutathione S-transferase alpha 4-like Protein, glutathione S-transferase alpha 4 S homeolog Protein, glutathione S-transferase 3 Protein, glutathione S-transferase, alpha 4 Protein, glutathione S-transferase A4 Protein, GSTA4 Protein, Gsta4 Protein, GSTA4L Protein, gsta4 Protein, gsta4.S Protein, LOC100341622 Protein

Hintergrund: GSTA4, also known as glutathione S-transferase A4, belongs to the GST superfamily. This enzyme is involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. GSTA4 shows very high activity with reactive carbonyl compounds such as alk-2-enals. GSTA4 is highly effective in catalyzing the conjugate addition of reduced glutathione to 4-hydroxynonenal, an important product of peroxidative degradation of arachidonic acid and a commonly used biomarker for oxidative damage in tissue. This enzyme is expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver. Recombinant human GSTA4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 28.3kDa (246aa) confirmed by MALDI-TOF

NCBI Accession: NP_001503

UniProt: O15217