IMPAD1 Protein (AA 34-359) (His tag)

Produktnummer ABIN6387553

Produktdetails

Target: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 34-359

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses IMPAD1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific acitivty > 3300 pmole/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4)

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))

Andere Bezeichnung: IMPAD1 (IMPAD1 Produkte)

Synonyme: IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein

Hintergrund: IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molekulargewicht: 37.6kDa (349aa) confirmed by MALDI-TOF

NCBI Accession: NP_060283

UniProt: Q9NX62

Pathways: Glycosaminoglycan Metabolic Process