HCV NS5a Protein (AA 2210-2306) (His tag)

Produktnummer ABIN6387451

Produktdetails

Target: HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2210-2306

Spezies: Hepatitis C Virus (HCV)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses HCV NS5a Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MSLKATCTAN HDSPDAELIE ANLLWRQEMG GNITRVESEN KVVILDSFDP LVAEEDEREV SVPAEILRKS RRFARALPVW ARPDYNPPLV ETWKKPDY

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.2M NaCl, 40 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: HCV NS5a (Hepatitis C Virus NS5a (HCV NS5a))

Andere Bezeichnung: HCV-NS5a (HCV NS5a Produkte)

Substanzklasse: Viral Protein

Hintergrund: NS5A is a component of the replication complex involved in RNA-binding. Its interaction with Human VAPB may target the viral replication complex to vesicles. This protein down-regulates viral IRES translation initiation and mediates interferon resistance, presumably by interacting with and inhibiting human EIF2AK2/PKR. NS5a seems to inhibit apoptosis by interacting with BIN1 and FKBP8. The hyperphosphorylated form of NS5A is an inhibitor of viral replication. Recombinant HCV(Hepatitis C Virus) NS5A protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 13.4 kDa (118aa) confirmed by MALDI-TOF

NCBI Accession: NP_671491