YOD1 Protein (His tag)

Produktnummer ABIN1098573

Produktdetails

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses YOD1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Aufreinigung: purified by chromatography

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Kommentare:

Synonyms: Ubiquitin thioesterase OTU1, DUBA8, OTUD2, PRO0907, RP11-164O23.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 30% glycerol, 1 MM DTT

Lagerung: 4 °C

Informationen zur Lagerung: Avoid repeated freezing and thawing cycles.

Antigendetails

Target: YOD1 (Ubiquitin Thioesterase OTU1 (YOD1))

Andere Bezeichnung: YOD1 (YOD1 Produkte)

Synonyme: DUBA8 Protein, OTUD2 Protein, PRO0907 Protein, hshin7 Protein, wu:fe01a10 Protein, zgc:112182 Protein, 9930028C20Rik Protein, Hshin7 Protein, YOD1 deubiquitinase Protein, YOD1 deubiquitinase L homeolog Protein, YOD1 Protein, Yod1 Protein, yod1.L Protein, yod1 Protein

Hintergrund: YOD1 is a highly conserved deubiquitinating enzyme of the ovarian tumor (otubain) family, whose function has yet to be assigned in mammalian cells. YOD1 is a constituent of a multiprotein complex with p97 as its nucleus, suggesting a functional link to a pathway responsible for the dislocation of misfolded proteins from the endoplasmic reticulum. Expression of a YOD1 variant deprived of its deubiquitinating activity imposes a halt on the dislocation reaction, as judged by the stabilization of various dislocation substrates. Recombinant human YOD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 40.7 kDa (371aa) confirmed by MALDI-TOF

NCBI Accession: NP_061036

Pathways: ER-Nucleus Signaling