EGLN3 Protein (His tag)
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- Target Alle EGLN3 Proteine anzeigen
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses EGLN3 Protein ist gelabelt mit His tag.
- Applikation
- SDS-PAGE (SDS)
- Aufreinigung
- purified by using conventional chromatography.
- Top Product
- Discover our top product EGLN3 Protein
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Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (His tag)
EGLN3 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 1-239) protein (GST tag)EGLN3 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 94-193) protein (GST tag)EGLN3 Spezies: Human Wirt: Wheat germ Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) (AA 2-239) protein (His tag)EGLN3 Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 90 % SDS
Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3) protein (Myc-DYKDDDDK Tag)EGLN3 Spezies: Human Wirt: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Kommentare
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Synonyms: Egl nine homolog 3, HIFPH3, PHD3
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.3M NaCl, 5 MM DTT, 2 mM EDTA
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Avoid repeated freezing and thawing cycles.
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- Target
- EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))
- Andere Bezeichnung
- EGLN3 (EGLN3 Produkte)
- Synonyme
- zgc:77019 Protein, wu:fj78a08 Protein, EGLN3 Protein, phd3 Protein, PHD-3 Protein, PHD3 Protein, SM-20 Protein, 2610021G09Rik Protein, AI505553 Protein, AI648162 Protein, Hif-p4h-3 Protein, Phd3 Protein, HIFP4H3 Protein, HIFPH3 Protein, egl-9 family hypoxia-inducible factor 3 Protein, egl-9 family hypoxia inducible factor 3 Protein, egl-9 family hypoxia-inducible factor 3 L homeolog Protein, egln3 Protein, EGLN3 Protein, egln3.L Protein, Egln3 Protein
- Hintergrund
- EGLN3, a member of the EGLN family of prolyl hydroxylases, has been shown to catalyze hydroxylation of the alpha subunit of hypoxia-inducible factor-alpha, which targets hypoxia-inducible factor-alpha for ubiquitination by a ubiquitin ligase complex containing the von Hippel-Lindau (VHL) tumor suppressor. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM2 in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Recombinant human EGLN3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molekulargewicht
- 29.8 kDa (263aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_071356
- Pathways
- Positive Regulation of Endopeptidase Activity
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