REG3B Protein (AA 27-175) (His tag)

Produktnummer ABIN6239917

Produktdetails

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Biologische Aktivität: Active

Proteineigenschaft: AA 27-175

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses REG3B Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 97 %

Biological Activity Comment: Regenerating Islet Derived Protein 3 Beta (REG3b) also known as PAP-I and HIP is bactericidal C-type lectin which acts against several intestinal Gram-positive bacteria and Gram-negative bacteria. The Reg family proteins have been implicated in a range of physiological processes including acting as acute phase reactants, lectins, survival/growth factors for insulin-producing pancreatic beta-cells, neural cells, and epithelial cells of the digestive system. To test the effect of REG3b on cell proliferation of SK-N-SH, SK-N-SH cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of REG3b. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .Cell proliferation of SK-N-SH cells after incubation with REG3b for 72h observed by inverted microscope was shown in Figure 1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 7.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: REG3B (Regenerating Islet-Derived 3 beta (REG3B))

Andere Bezeichnung: Regenerating Islet Derived Protein 3 Beta (REG3b) (REG3B Produkte)

Synonyme: HIP Protein, PAP1 Protein, Pap Protein, REG-III Protein, Pap1 Protein, regenerating islet-derived 3 beta Protein, regenerating family member 3 beta Protein, Reg3b Protein

Hintergrund: Alternative Names: PAP1, Pancreatitis-Associated Protein 1

Molekulargewicht: 17kDa

UniProt: P35230