APRT Protein (AA 1-180) (His tag)

Produktnummer ABIN5665757

Produktdetails

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Biologische Aktivität: Active

Proteineigenschaft: AA 1-180

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses APRT Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 97 %

Biological Activity Comment: Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of APRT with VCAM1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 6.8

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Abstract: APRT Produkte

Synonyme: Tb07.43M14.200 Protein, Tb07.43M14.180 Protein, C85684 Protein, AMP Protein, APRTD Protein, adenine phosphoribosyltransferase Protein, adenine phosphoribosyl transferase Protein, CND05020 Protein, Tb927.7.1790 Protein, Tb927.7.1780 Protein, Arnit_0941 Protein, Saut_1231 Protein, Fbal_1180 Protein, PH_RS07970 Protein, PF_RS08760 Protein, PAB_RS02560 Protein, Aprt Protein, APRT Protein

Hintergrund: Alternative Names: AMP, APRTase

Molekulargewicht: 23kDa

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process