GSTM1 Protein (AA 1-218) (His tag)

Produktnummer ABIN6239877

Produktdetails

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Biologische Aktivität: Active

Proteineigenschaft: AA 1-218

Spezies: Ratte

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GSTM1 Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GSTM1 with UCP1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 8.3

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))

Andere Bezeichnung: Glutathione S Transferase Mu 1 (GSTm1) (GSTM1 Produkte)

Synonyme: GST1 Protein, GSTM1-1 Protein, GSTM1a-1a Protein, GSTM1b-1b Protein, GTH4 Protein, GTM1 Protein, H-B Protein, MU Protein, MU-1 Protein, GSTA3 Protein, Gstb-1 Protein, Gstb1 Protein, gst-mu Protein, gst1 Protein, gstm1 Protein, gstm2 Protein, gth4 Protein, gtm1 Protein, glutathione S-transferase mu 1 Protein, glutathione S-transferase M1 Protein, glutathione S-transferase, mu 1 Protein, glutathione S-transferase Mu 1 Protein, glutathione S-transferase mu 2 (muscle) Protein, glutathione S-transferase mu 1 L homeolog Protein, GSTM1 Protein, Gstm1 Protein, LOC100356307 Protein, GSTM2 Protein, LOC100731426 Protein, gstm1.L Protein

Hintergrund: Alternative Names: GST-M1, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU1, GST HB subunit 4

Molekulargewicht: 27kDa

UniProt: P04905

Pathways: Negative Regulation of Transporter Activity