Adiponectin Receptor 1 Protein (ADIPOR1) (AA 1-136) (His tag,GST tag)

Produktnummer ABIN5665758

Produktdetails

Target: Adiponectin Receptor 1 (ADIPOR1)

Biologische Aktivität: Active

Proteineigenschaft: AA 1-136

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses Adiponectin Receptor 1 Protein ist gelabelt mit His tag,GST tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His and GST Tag

Reinheit: > 97 %

Biological Activity Comment: ADIPOR1 (Adiponectin receptor protein 1) is a receptor for ADP (30kDa adipocyte complement-related protein), an essential hormone secreted by adipocytes that regulates glucose and lipid metabolism. ADIPOQ-binding activates a signaling cascade that leads to increased AMPK activity, and ultimately to increased fatty acid oxidation, increased glucose uptake and decreased gluconeogenesis. Besides, human ADP and rat ADP exist similarities in amino acid sequence with the identity of 83.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ADIPOR1 and recombinant rat ADP. Briefly, ADIPOR1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ADP-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ADIPOR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ADIPOR1 and ADP was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ADIPOR1 with ADP.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.4

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: Adiponectin Receptor 1 (ADIPOR1)

Abstract: ADIPOR1 Produkte

Synonyme: ADIPOR1 Protein, adipor1 Protein, zgc:92893 Protein, ACDCR1 Protein, CGI45 Protein, PAQR1 Protein, TESBP1A Protein, 2810031L11Rik Protein, CGI-45 Protein, Paqr1 Protein, zgc:55386 Protein, wu:fj02d08 Protein, acdcr1 Protein, cgi-45 Protein, cgi45 Protein, paqr1 Protein, tesbp1a Protein, MGC53458 Protein, Adipor1 Protein, adiponectin receptor 1 Protein, adiponectin receptor 1a Protein, adiponectin receptor 1b Protein, adiponectin receptor 1 S homeolog Protein, adiponectin receptor 1 L homeolog Protein, ADIPOR1 Protein, adipor1a Protein, Adipor1 Protein, adipor1b Protein, adipor1 Protein, adipor1.S Protein, adipor1.L Protein

Hintergrund: Alternative Names: ACDCR1, CGI45, PAQR1, TESBP1A, TESBP1-A, Progestin and AdipoQ Receptor Family Member I

Molekulargewicht: 63kDa

UniProt: Q96A54

Pathways: AMPK Signaling