CIRBP Protein (AA 1-172) (His tag)

Produktnummer ABIN6239789

Produktdetails

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Biologische Aktivität: Active

Proteineigenschaft: AA 1-172

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CIRBP Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 95 %

Biological Activity Comment: CIRBP (Cold-inducible RNA-binding protein) is considered to play a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival and act as a translational activator. Besides, ATXN1 (Ataxin-1) has been proven as an interactor of CIRBP. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CIRBP and recombinant human ATXN1. Briefly, CIRBP were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ATXN1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-CIRBP mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of CIRBP and ATXN1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of CIRBP with ATXN1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 9.7

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: CIRBP (Cold Inducible RNA Binding Protein (CIRBP))

Abstract: CIRBP Produkte

Synonyme: CIRP Protein, Cirp Protein, R74941 Protein, Xcirp Protein, cirbp Protein, cirbp-a Protein, cirbp2 Protein, cirp Protein, cirp1 Protein, cirp2 Protein, xcirp2 Protein, XCIRP Protein, XCIRP-1 Protein, cirbp-b Protein, cirp-1 Protein, APBP-1 Protein, zgc:136559 Protein, CIRBP Protein, A18 hnRNP Protein, cold inducible RNA binding protein Protein, cold-inducible RNA-binding protein Protein, cold inducible RNA binding protein S homeolog Protein, cold inducible RNA binding protein L homeolog Protein, cold inducible RNA binding protein b Protein, CIRBP Protein, EDI_107320 Protein, Cirbp Protein, cirbp.S Protein, cirbp.L Protein, cirbp Protein, cirbpb Protein

Hintergrund: Alternative Names: CIRP, A18HNRNP, Glycine-Rich RNA Binding Protein

Molekulargewicht: 22/27kDa

UniProt: Q14011

Pathways: Ribonucleoprotein Complex Subunit Organization