LMTK3 Protein (AA 133-411) (His tag)
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- Target Alle LMTK3 Proteine anzeigen
- LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 133-411
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- Dieses LMTK3 Protein ist gelabelt mit His tag.
- Applikation
- Activity Assay (AcA), Cell Culture (CC)
- Produktmerkmale
- Tag location: N-terminal His Tag
- Reinheit
- > 95 %
- Biological Activity Comment
- Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.
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- Applikationshinweise
- Isoelectric Point: 6.3
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- Konservierungsmittel
- Dithiothreitol (DTT), Other preservative, ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- Target
- LMTK3 (Lemur Tyrosine Kinase 3 (LMTK3))
- Abstract
- LMTK3 Produkte
- Synonyme
- LMTK3 Protein, LMR3 Protein, TYKLM3 Protein, BC059845 Protein, aatyk3 Protein, lemur tyrosine kinase 3 Protein, lmtk3 Protein, LMTK3 Protein, Lmtk3 Protein
- Hintergrund
- Alternative Names: LMR3, TYKLM3, Serine/threonine-protein kinase LMTK3
- Molekulargewicht
- 46kDa
- UniProt
- Q96Q04
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