CSTF1 Protein (AA 1-431) (His tag)

Produktnummer ABIN5854876

Produktdetails

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-431

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses CSTF1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH

Reinheit: > 90 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 1  mM DTT, 20 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))

Andere Bezeichnung: CSTF1 (CSTF1 Produkte)

Synonyme: fc22d10 Protein, zgc:66207 Protein, zgc:77560 Protein, wu:fc22d10 Protein, wu:fd16h09 Protein, MGC53063 Protein, MGC69421 Protein, MGC83790 Protein, 1700057K18Rik Protein, AI788832 Protein, CstF-50 Protein, CstFp50 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1 Protein, cleavage stimulation factor subunit 1 Protein, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa Protein, cstf1 Protein, cstf1.S Protein, CSTF1 Protein, cstf1.L Protein, Cstf1 Protein

Hintergrund: CSTF1, also known as cleavage stimulation factor subunit 1, is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. It is functioning with cleavage-polyadenylation specificity factor, two cleavage factors, and poly(A)+ polymerase. It is involved in the 3' end processing of messenger RNA precursors. Recombinant human CSTF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 49.1kDa (437aa) 40-57kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_001028693

UniProt: Q05048