Carboxylesterase 2E (CES2E) (AA 27-559) (Active) protein (His tag)

Produktnummer ABIN5854477

Produktdetails

Target: Carboxylesterase 2E (CES2E)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 27-559

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: His tag

Applikation: SDS-PAGE (SDS)

Sequenz: QDSASPIRNT HTGQVRGSLV HVKDTDIAVH TFLGIPFAKP PVGPLRFAPP EAPEPWSGVR DGTSHPNMCL QNDNLMGSED LKMMNLILPP ISMSEDCLYL NIYVPAHAHE GSNLPVMVWI HGGALTVGMA SMYDGSMLAA TEDVVVVAIQ YRLGVLGFFS TGDQHAKGNW GYLDQVAALR WVQQNIVHFG GNPDRVTIFG ESAGGTSVSS HVVSPMSQGL FHGAIMESGV AVLPDLISSS SEMVHRIVAN LSGCAAVNSE TLMCCLRGKN EAEMLAINKV FKIIPGVVDG EFLPKHPQEL MASKDFHPVP SIIGINNDEY GWILPTIMDP AQKIEEITRK TLPAVLKSTA LKMMLPPECG DLLMEEYMGD TEDPETLQAQ FREMKGDFMF VIPALQVAHF QRSHAPVYFY EFQHRPSFFK DFRPPYVKAD HGDEIFLVFG YQFGNIKLPY TEEEEQLSRR IMKYWANFAR HGNPNSEGLP YWPVMDHDEQ YLQLDIQPSV GRALKARRLQ FWTKTLPQKI QELKGSQERH KELLEHHHHH H

Reinheit: > 95 % by SDS - PAGE

Endotoxin-Niveau: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In Phosphate Buffered Saline (  pH 7.4) containing 10 % glycerol.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: Carboxylesterase 2E (CES2E)

Andere Bezeichnung: Pyrethroid hydrolase Ces2e (CES2E Produkte)

Synonyme: 9030624L02Rik Protein, Ces5 Protein, carboxylesterase 2E Protein, Ces2e Protein

Hintergrund: CES2E, also known as pyrethroid hydrolase Ces2e, is a type of enzyme that capable of hydrolyzing a variety of carboxylic acid esters and it is widely distributed in cells especially in mammalian liver. It is involved in the chemical reaction, exerting its role in catalyzing the carboxylic ester and water to convert to an alcohol and a carboxylate. Recombinant mouse CES2E, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molekulargewicht: 60.5kDa (541aa) 50-70kDa (SDS-PAGE under reducing conditions)

NCBI Accession: NP_766347

UniProt: Q8BK48