GSTA1 Protein (AA 1-223) (His tag)

Produktnummer ABIN5854233

Produktdetails

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-223

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GSTA1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMAGKPVL HYFNARGRME CIRWLLAAAG VEFEEKFIQS PEDLEKLKKD GNLMFDQVPM VEIDGMKLAQ TRAILNYIAT KYDLYGKDMK ERALIDMYSE GILDLTEMIG QLVLCPPDQR EAKTALAKDR TKNRYLPAFE KVLKSHGQDY LVGNRLTRVD IHLLEVLLYV EEFDASLLTP FPLLKAFKSR ISSLPNVKKF LQPGSQRKPP MDAKQIQEAR KAFKIQ

Reinheit: > 95 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 4,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 pmole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Andere Bezeichnung: Gsta1 (GSTA1 Produkte)

Synonyme: GST2 Protein, GSTA1-1 Protein, GTH1 Protein, Gst2-1 Protein, OTTMUSG00000031890 Protein, GSTA3 Protein, GST Protein, GSTA1 Protein, gst2 Protein, gth1 Protein, gsta1-1 Protein, Gsta1 Protein, Gst-2 Protein, Gst-3 Protein, Gst2 Protein, Gst3 Protein, glutathione S-transferase alpha 1 Protein, glutathione S-transferase, alpha 1 (Ya) Protein, glutathione S-transferase alpha 3 Protein, glutathione S-transferase alpha 5 Protein, glutathione S-transferase alpha 1 L homeolog Protein, glutathione S-transferase alpha 2 Protein, glutathione S-transferase mu 2 (muscle) Protein, Glutathione S-transferase 2 Protein, glutathione S-transferase A Protein, glutathione S-transferase Gst2 Protein, glutathione S-transferase 2 Protein, GSTA1 Protein, Gsta1 Protein, GSTA3 Protein, GSTA5 Protein, gsta1.L Protein, gsta1 Protein, Gsta2 Protein, GSTM2 Protein, gst-2 Protein, LOC100713029 Protein, gst2 Protein, LOC101897277 Protein

Hintergrund: Gsta1 also known as Glutathione S-transferase, A1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant mouse Gsta1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 28kDa (246aa) confirmed by MALDI-TOF

NCBI Accession: NP_032207

UniProt: P13745