CA13 Protein (AA 1-262) (His tag)

Produktnummer ABIN5854197

Produktdetails

Target: CA13 (Carbonic Anhydrase XIII (CA13))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 1-262

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CA13 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSP MGSMSRLSWG YREHNGPIHW KEFFPIADGD QQSPIEIKTK EVKYDSSLRP LSIKYDPSSA KIISNSGHSF NVDFDDTENK SVLRGGPLTG SYRLRQVHLH WGSADDHGSE HIVDGVSYAA ELHVVHWNSD KYPSFVEAAH EPDGLAVLGV FLQIGEPNSQ LQKITDTLDS IKEKGKQTRF TNFDLLSLLP PSWDYWTYPG SLTVPPLLES VTWIVLKQPI NISSQQLAKF RSLLCTAEGE AAAFLVSNHR PPQPLKGRKV RASFH

Reinheit: > 90 % by SDS - PAGE

Biological Activity Comment: Specific activity is > 2,500 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Bioactivity Validated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid, In Phosphate buffered saline (  pH 7.4) containing 10 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: CA13 (Carbonic Anhydrase XIII (CA13))

Andere Bezeichnung: CA13 (CA13 Produkte)

Synonyme: 2310075C21Rik Protein, Ca13 Protein, CAXIII Protein, car13 Protein, RGD1560453 Protein, car3 Protein, CA13 Protein, carbonic anhydrase 13 Protein, carbonic anhydrase XIII L homeolog Protein, carbonic anhydrase XIII Protein, Car13 Protein, CA13 Protein, ca13.L Protein, ca13 Protein, LOC100152714 Protein, Ca13 Protein, LOC101113860 Protein

Hintergrund: CA13 also known as carbonic anhydrase 13 belongs to the alpha-carbonic anhydrase family. The carbonic anhydrase from a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that occurs relatively slowly in the absence of catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are claasified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta, and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Recombinant human CA13, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 31.8kDa (285aa) confirmed by MALDI-TOF

NCBI Accession: NP_940986

UniProt: Q8N1Q1