ATP6V1F Protein (AA 1-119) (His tag)

Produktnummer ABIN5854054

Produktdetails

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-119

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ATP6V1F Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMAGRGKL IAVIGDEDTV TGFLLGGIGE LNKNRHPNFL VVEKDTTINE IEDTFRQFLN RDDIGIILIN QYIAEMVRHA LDAHQQSIPA VLEIPSKEHP YDAAKDSILR RARGMFTAED LR

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In Phosphate buffered saline (  pH 7.4) containing, 50 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ATP6V1F (ATPase, H+ Transporting, Lysosomal 14kDa, V1 Subunit F (ATP6V1F))

Andere Bezeichnung: ATP6V1F (ATP6V1F Produkte)

Synonyme: Atp6s14 Protein, Vma7 Protein, atp6s14 Protein, atp6v1f Protein, vatf Protein, ATP6S14 Protein, VATF Protein, 1110004G16Rik Protein, ATPase H+ transporting V1 subunit F Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F S homeolog Protein, ATPase, H+ transporting, lysosomal 14kDa, V1 subunit F L homeolog Protein, ATPase, H+ transporting, lysosomal V1 subunit F Protein, Atp6v1f Protein, atp6v1f.S Protein, ATP6V1F Protein, atp6v1f.L Protein

Hintergrund: ATP6V1F is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. Recombinant human ATP6V1F, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15.8kDa (142aa) confirmed by MALDI-TOF

NCBI Accession: NP_004222

UniProt: Q16864

Pathways: Transition Metal Ion Homeostasis, Proton Transport