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RNGTT Protein (AA 1-597) (His tag)
RNGTT
Spezies: Human
Wirt: Escherichia coli (E. coli)
Recombinant
> 85 % by SDS - PAGE
SDS
Produktnummer ABIN5853868
Produktdetails
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Target
Alle RNGTT Proteine anzeigen
RNGTT
(RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
Protein-Typ
Recombinant
Proteineigenschaft
AA 1-597
Spezies
Alle Spezies für RNGTT Proteine
Human
Quelle
Alle Expressionssysteme für RNGTT Proteine
Escherichia coli (E. coli)
Aufreinigungstag / Konjugat
Dieses RNGTT Protein ist gelabelt mit His tag.
Applikation
SDS-PAGE (SDS)
Sequenz
MGSSHHHHHH SSGLVPRGSH MGSMAHNKIP PRWLNCPRRG QPVAGRFLPL KTMLGPRYDS QVAEENRFHP SMLSNYLKSL KVKMGLLVDL TNTSRFYDRN DIEKEGIKYI KLQCKGHGEC PTTENTETFI RLCERFNERN PPELIGVHCT HGFNRTGFLI CAFLVEKMDW SIEAAVATFA QARPPGIYKG DYLKELFRRY GDIEEAPPPP LLPDWCFEDD EDEDEDEDGK KESEPGSSAS FGKRRKERLK LGAIFLEGVT VKGVTQVTTQ PKLGEVQQKC HQFCGWEGSG FPGAQPVSMD KQNIKLLDLK PYKVSWKADG TRYMMLIDGT NEVFMIDRDN SVFHVSNLEF PFRKDLRMHL SNTLLDGEMI IDRVNGQAVP RYLIYDIIKF NSQPVGDCDF NVRLQCIERE IISPRHEKMK TGLIDKTQEP FSVRNKPFFD ICTSRKLLEG NFAKEVSHEM DGLIFQPTGK YKPGRCDDIL KWKPPSLNSV DFRLKITRMG GEGLLPQNVG LLYVGGYERP FAQIKVTKEL KQYDNKIIEC KFENNSWVFM RQRTDKSFPN AYNTAMAVCN SISNPVTKEM LFEFIDRCTA ASQGQKRKHH LDPDTELMPP PPPKRPRPLT
Reinheit
> 85 % by SDS - PAGE
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Alternativen
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Format
Liquid
Konzentration
0.25 mg/mL
Buffer
Liquid. In 20 mM Tris-HCl buffer ( pH 7.5) containing 0.2M NaCl, 40 % glycerol, 2 mM DTT, 0.1 mM PMSF
Lagerung
4 °C,-20 °C,-80 °C
Informationen zur Lagerung
Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Antigendetails
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Target
RNGTT
(RNA Guanylyltransferase and 5'-Phosphatase (RNGTT))
Andere Bezeichnung
RNGTT (RNGTT Produkte )
Synonyme
xcap1b Protein, MGC75787 Protein, CAP1A Protein, HCE Protein, HCE1 Protein, hCAP Protein, AU020997 Protein, zgc:76886 Protein, RNA guanylyltransferase and 5'-phosphatase Protein, mRNA-capping enzyme Protein, rngtt.S Protein, rngtt Protein, RNGTT Protein, LOC100439097 Protein, Rngtt Protein
Hintergrund
RNGTT is a bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. It catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. Recombinant human RNGTT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Molekulargewicht
70.9 kDa (620aa)
NCBI Accession
NP_003791
UniProt
O60942
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