ZWILCH Protein (AA 1-591) (His tag)

Produktnummer ABIN5853741

Produktdetails

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-591

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses ZWILCH Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMWERLNC AAEDFYSRLL QKFNEEKKGI RKDPFLYEAD VQVQLISKGQ PNPLKNILNE NDIVFIVEKV PLEKEETSHI EELQSEETAI SDFSTGENVG PLALPVGKAR QLIGLYTMAH NPNMTHLKIN LPVTALPPLW VRCDSSDPEG TCWLGAELIT TNNSITGIVL YVVSCKADKN YSVNLENLKN LHKKRHHLST VTSKGFAQYE LFKSSALDDT ITASQTAIAL DISWSPVDEI LQIPPLSSTA TLNIKVESGE PRGPLNHLYR ELKFLLVLAD GLRTGVTEWL EPLEAKSAVE LVQEFLNDLN KLDGFGDSTK KDTEVETLKH DTAAVDRSVK RLFKVRSDLD FAEQLWCKMS SSVISYQDLV KCFTLIIQSL QRGDIQPWLH SGSNSLLSKL IHQSYHGTMD TVSLSGTIPV QMLLEIGLDK LKKDYISFFI GQELASLNHL EYFIAPSVDI QEQVYRVQKL HHILEILVSC MPFIKSQHEL LFSLTQICIK YYKQNPLDEQ HIFQLPVRPT AVKNLYQSEK PQKWRVEIYS GQKKIKTVWQ LSDSSPIDHL NFHKPDFSEL TLNGSLEERI FFTNMVTCSQ VHFK

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.25 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.1M NaCl, 20 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: ZWILCH (Zwilch, Kinetochore Associated, Homolog (ZWILCH))

Andere Bezeichnung: ZWILCH (ZWILCH Produkte)

Synonyme: CG18639 Protein, CG18729 Protein, Dmel\\CG18729 Protein, Zwilch Protein, hzwilch Protein, kntc1ap Protein, KNTC1AP Protein, hZwilch Protein, 2310031L18Rik Protein, si:ch211-242m18.4 Protein, wu:fb10f09 Protein, zwilch kinetochore protein Protein, CG18729 gene product from transcript CG18729-RA Protein, protein zwilch homolog Protein, Zwilch Protein, zwilch Protein, LOC100374963 Protein, ZWILCH Protein

Hintergrund: ZWILCH belongs to the ZWILCH family. This protein is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. It is required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Recombinant human ZWILCH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 69.6kDa(614aa)

NCBI Accession: NP_060445

UniProt: Q9H900