EIF3I Protein (AA 1-325) (His tag)

Produktnummer ABIN5853326

Produktdetails

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-325

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses EIF3I Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMKPILLQ GHERSITQIK YNREGDLLFT VAKDPIVNVW YSVNGERLGT YMGHTGAVWC VDADWDTKHV LTGSADNSCR LWDCETGKQL ALLKTNSAVR TCGFDFGGNI IMFSTDKQMG YQCFVSFFDL RDPSQIDNNE PYMKIPCNDS KITSAVWGPL GECIIAGHES GELNQYSAKS GEVLVNVKEH SRQINDIQLS RDMTMFVTAS KDNTAKLFDS TTLEHQKTFR TERPVNSAAL SPNYDHVVLG GGQEAMDVTT TSTRIGKFEA RFFHLAFEEE FGRVKGHFGP INSVAFHPDG KSYSSGGEDG YVRIHYFDPQ YFEFEFEA

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Denatured

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer: Liquid. 20  mM Tris-HCl buffer (  pH 8.0) containing 10 % glycerol 0.4M urea

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: EIF3I (Eukaryotic Translation Initiation Factor 3, Subunit I (EIF3I))

Andere Bezeichnung: EIF3I (EIF3I Produkte)

Synonyme: EIF3S2 Protein, PRO2242 Protein, TRIP-1 Protein, TRIP1 Protein, eIF3-beta Protein, eIF3-p36 Protein, 36kDa Protein, D4Ertd632e Protein, Eif3s2 Protein, Trip1 Protein, eIF-3-beta Protein, eIF3 p36 Protein, eif3s2 Protein, hm:zehn0464 Protein, wu:fb62b08 Protein, zgc:56211 Protein, zgc:77823 Protein, CaO19.2967 Protein, eIF-3 39 kDa subunit homolog Protein, eIF3i Protein, eif3-beta Protein, eif3-p36 Protein, eif3i Protein, pro2242 Protein, trip-1 Protein, trip1 Protein, eukaryotic translation initiation factor 3 subunit I Protein, eukaryotic translation initiation factor 3, subunit I Protein, Eukaryotic Initiation Factor Protein, Eukaryotic translation initiation factor 3 subunit I Protein, translation initiation factor eIF3 subunit i Protein, eukaryotic translation initiation factor 3 subunit I L homeolog Protein, EIF3I Protein, Eif3i Protein, eif-3.I Protein, eIF3i Protein, eif3i Protein, TIF34 Protein, eif3i.L Protein

Hintergrund: EIF3I is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AuG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3I protein, fused to His-tag at N-terminus, was expressed in E.coli.

Molekulargewicht: 38.9kDa (348aa)

NCBI Accession: NP_003748

UniProt: Q13347

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Ribonucleoprotein Complex Subunit Organization, Synthesis of DNA