MBP Protein (AA 1-197) (His tag)

Produktnummer ABIN5853266

Produktdetails

Target: MBP (Myelin Basic Protein (MBP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-197

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses MBP Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMGNHAGK RELNAEKAST NSETNRGESE KKRNLGELSR TTSEDNEVFG EADANQNNGT SSQDTAVTDS KRTADPKNAW QDAHPADPGS RPHLIRLFSR DAPGREDNTF KDRPSESDEL QTIQEDSAAT SESLDVMASQ KRPSQRHGSK YLATASTMDH ARHGFLPRHR DTGILDSIGR FFGGDRGAPK RGSGKVSSEE

Reinheit: > 85 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol,

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: MBP (Myelin Basic Protein (MBP))

Andere Bezeichnung: Myelin basic protein (MBP Produkte)

Synonyme: C76307 Protein, Hmbpr Protein, R75289 Protein, golli-mbp Protein, mld Protein, shi Protein, Mbps Protein, cb274 Protein, fj33b11 Protein, mbp Protein, wu:fj33b11 Protein, wu:fq15b02 Protein, zgc:136630 Protein, MGC115135 Protein, myelin basic protein Protein, myelin basic protein a Protein, myelin basic protein L homeolog Protein, MBP Protein, Mbp Protein, mbpa Protein, mbp Protein, mbp.L Protein

Hintergrund: MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called 'Golli-MBP') that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. Recombinant human MBP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 23.9 kDa (220aa) confirmed by MALDI-TOF

NCBI Accession: NP_001020271

UniProt: P02686