SPA17 Protein (AA 1-151) (His tag)

Produktnummer ABIN5852921

Produktdetails

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-151

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SPA17 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: MGSSHHHHHH SSGLVPRGSH MGSMSIPFSN THYRIPQGFG NLLEGLTREI LREQPDNIPA FAAAYFESLL EKREKTNFDP AEWGSKVEDR FYNNHAFEEQ EPPEKSDPKQ EESQISGKEE ETSVTILDSS EEDKEKEEVA AVKIQAAFRG HIAREEAKKM KTNSLQNEEK EENK

Reinheit: > 90 % by SDS - PAGE

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid. In 20  mM Tris-HCl buffer (  pH 8.0) containing 0.15M NaCl, 10 % glycerol, 1  mM DTT

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Andere Bezeichnung: SPA17 (SPA17 Produkte)

Synonyme: spa17 Protein, MGC89454 Protein, SPA17 Protein, CT22 Protein, SP17 Protein, SP17-1 Protein, Sp17 Protein, sperm autoantigenic protein 17 Protein, sperm autoantigenic protein 17 L homeolog Protein, spa17 Protein, SPA17 Protein, spa17.L Protein, Spa17 Protein

Hintergrund: SPA17 is a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. Recombinant human SPA17 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molekulargewicht: 19.8 kDa (174aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)

NCBI Accession: NP_059121

UniProt: Q15506