NCAP (AA 1-168), (Extracellular) protein (GST tag)

Produktnummer ABIN5710994

Produktdetails

Target: NCAP

Protein-Typ: Recombinant

Proteineigenschaft: Extracellular, AA 1-168

Spezies: Influenza B Virus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: GST tag

Applikation: SDS-PAGE (SDS)

Sequenz: MSNMDIDGIN TGTIDKTPEE IISGTSGATR PIIRPATLAP PSNKRTRNPS PERATTSSEA DVGRKTQKKQ TPTEIKKSVY NMVVKLGEFY NQMMVKAGLN DDMERNLIQN AHAVERILLA ATDDKKTEFQ KKKNARDVKE GKEEIDHNKT GGTFYKMVRD DKTIYFSP

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: NCAP

Substanzklasse: Viral Protein

Hintergrund: Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as tplate for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP ses to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus .

Molekulargewicht: 46.2 kDa

UniProt: P04666