POLN Protein (AA 60-240) (His tag)

Produktnummer ABIN5710850

Produktdetails

Target: POLN (Polymerase (DNA Directed) nu (POLN))

Protein-Typ: Recombinant

Proteineigenschaft: AA 60-240

Spezies: Hepatitis E Virus (HEV)

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses POLN Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS)

Sequenz: EVFWNHPIQR VIHNELELYC RARSGRCLEI GAHPRSINDN PNVVHRCFLR PAGRDVQRWY TAPTRGPAAN CRRSALRGLP AADRTYCFDG FSGCNFPAET GIALYSLHDM SPSDVAEAMF RHGMTRLYAA LHLPPEVLLP PGTYRTASYL LIHDGRRVVV TYEGDTSAGY NHDVSNLRSW I

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: POLN (Polymerase (DNA Directed) nu (POLN))

Andere Bezeichnung: POLN (POLN Produkte)

Synonyme: POLN Protein, POL4P Protein, DNA polymerase nu Protein, polymerase (DNA directed) nu Protein, DNA polymerase N Protein, POLN Protein, Poln Protein

Substanzklasse: Viral Protein

Hintergrund: Methyltransferase displays a cytoplasmic capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m7GTP or m7GDP. GMP, GpppG, and GpppA were poor substrates for the methyltransferase. This enzyme also displays guanylyltransferase activity to form a covalent complex, methyltransferase-m7GMP, from which 7-methyl-GMP is transferred to the mRNA to create the cap structure. Cap analogs such as m7GTP, m7GDP, et2m7GMP, and m2et7GMP inhibit the methyltransferase reaction .RNA-directed RNA polymerase plays an essential role in the virus replication. Binds to the 3'-end of the genomic RNA, probably to initiate replication ..

Molekulargewicht: 24.5 kDa

UniProt: P33424