SRSF1 Protein (AA 2-248) (His-SUMO Tag)

Produktnummer ABIN5710356

Produktdetails

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-248

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses SRSF1 Protein ist gelabelt mit His-SUMO Tag.

Applikation: SDS-PAGE (SDS)

Sequenz: SGGGVIRGPA GNNDCRIYVG NLPPDIRTKD IEDVFYKYGA IRDIDLKNRR GGPPFAFVEF EDPRDAEDAV YGRDGYDYDG YRLRVEFPRS GRGTGRGGGG GGGGGAPRGR YGPPSRRSEN RVVVSGLPPS GSWQDLKDHM REAGDVCYAD VYRDGTGVVE FVRKEDMTYA VRKLDNTKFR SHEGETAYIR VKVDGPRSPS YGRSRSRSRS RSRSRSRSNS RSRSYSPRRS RGSPRYSPRH SRSRSRT

Aufreinigung: SDS-PAGE

Reinheit: > 90 %

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1-2 mg/mL

Buffer: 20 mM Tris-HCl based buffer, pH 8.0

Lagerung: -80 °C,4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Antigendetails

Target: SRSF1 (serine/arginine-Rich Splicing Factor 1 (SRSF1))

Andere Bezeichnung: SRSF1 (SRSF1 Produkte)

Synonyme: ASF Protein, SF2 Protein, SF2p33 Protein, SFRS1 Protein, SRp30a Protein, 1110054N12Rik Protein, 5730507C05Rik Protein, 6330415C05Rik Protein, AI482334 Protein, AW491331 Protein, Asf Protein, Sf2 Protein, Sfrs1 Protein, DKFZp469K2419 Protein, sfrs1 Protein, sfrs1b Protein, wu:fb80g05 Protein, zgc:111894 Protein, zgc:65898 Protein, zgc:76897 Protein, asf Protein, sf2 Protein, sf2p33 Protein, srp30a Protein, sfrs1a Protein, sfrs1l Protein, wu:fb52g10 Protein, wu:fb97g12 Protein, zgc:66146 Protein, serine and arginine rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1 Protein, serine/arginine-rich splicing factor 1b Protein, serine/arginine-rich splicing factor 1 L homeolog Protein, serine/arginine-rich splicing factor 1a Protein, SRSF1 Protein, Srsf1 Protein, srsf1b Protein, srsf1.L Protein, srsf1a Protein

Hintergrund: Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Molekulargewicht: 43.6 kDa

UniProt: Q07955

Pathways: Ribonucleoprotein Complex Subunit Organization